Research Areas

Cardiac

Publications

Gurung B, Tse G, Keung W, Li RA, Wong WT. Arrhythmic Risk Assessment of Hypokalaemia Using Human Pluripotent Stem Cell-Derived Cardiac Anisotropic Sheets. Front Cell Dev Biol. 2021 Dec 6;9:681665. doi: 10.3389/fcell.2021.681665. eCollection 2021.

Yuan S, Yin X, Meng X, Chan JF, Ye ZW, Riva L, Pache L, Chan CC, Lai PM, Chan CC, Poon VK, Lee AC, Matsunaga N, Pu Y, Yuen CK, Cao J, Liang R, Tang K, Sheng L, Du Y, Xu W, Lau CY, Sit KY, Au WK, Wang R, Zhang YY, Tang YD, Clausen TM, Pihl J, Oh J, Sze KH, Zhang AJ, Chu H, Kok KH, Wang D, Cai XH, Esko JD, Hung IF, Li RA, Chen H, Sun H, Jin DY, Sun R, Chanda SK, Yuen KY. Clofazimine broadly inhibits coronaviruses including SARS-CoV-2. Nature. 2021 May;593(7859):418-423. doi: 10.1038/s41586-021-03431-4. Epub 2021 Mar 16.

Wong AO, Gurung B, Wong WS, Mak SY, Tse WW, Li CM, Lieu DK, Costa KD, Li RA, Hajjar RJ. Adverse effects of hydroxychloroquine and azithromycin on contractility and arrhythmogenicity revealed by human engineered cardiac tissues. J Mol Cell Cardiol. 2021 Apr;153:106-110. doi: 10.1016/j.yjmcc.2020.12.014. Epub 2020 Dec 27.PMID: 33373642.

Sullivan S, Ginty P, McMahon S, May M, Solomon SL, Kurtz A, Stacey GN, Bennaceur Griscelli A, Li RA, Barry J, Song J, Turner ML. The Global Alliance for iPSC Therapies (GAiT). Stem Cell Res. 2020 Dec;49:102036. doi: 10.1016/j.scr.2020.102036. Epub 2020 Oct 22. PMID: 33212350.

Lee J, Mehrotra S, Zare-Eelanjegh E, Rodrigues RO, Akbarinejad A, Ge D, Amato L, Kiaee K, Fang Y, Rosenkranz A, Keung W, Mandal BB, Li RA, Zhang T, Lee H, Dokmeci MR, Zhang YS, Khademhosseini A, Shin S.R. A Heart-Breast Cancer-on-a-Chip Platform for Disease Modeling and Monitoring of Cardiotoxicity Induced by Cancer Chemotherapy. Small. 2020 Oct 23:e2004258. doi: 10.1002/smll.202004258.

Lam YY, Keung W, Chan CH, Geng L, Wong N, Brenière-Letuffe D, Li RA, Cheung YF. Single‐Cell Transcriptomics of Engineered Cardiac Tissues From Patient‐Specific Induced Pluripotent Stem Cell–Derived Cardiomyocytes Reveals Abnormal Developmental Trajectory and Intrinsic Contractile Defects in Hypoplastic Right Heart Syndrome. Journal of the American Heart Association. 2020 Oct 16; 9(20):e016528. doi: 10.1161/JAHA.120.016528.

Cheung YF, LI VW, Lai CT, Shin VY, Keung W, Cheuk DK, Kwong A, Li RA, Chan GC. Circulating high-sensitivity troponin T and microRNAs as markers of myocardial damage during childhood leukaemia treatment. Pediatr Res. 2020 Jul 7. doi: 10.1038/s41390-020-1049-5.

Wu MY, Leung JK, Liu L, Kam C. Chan KY, Li RA, Feng S, Chen S. A Small-Molecule AIE Chromosome Periphery Probe for Cytogenetic Studies. Angew Chem Int Ed Engl. 2020 Mar 12; 59(26):10327-10331. doi: 10.1002/anie.201916718.

Wong AO, Wong N, Geng L, Chow MZ, Lee EK, Wu H, Khine M, Kong CW, Costa KD, Keung W, Cheung YF, Li RA. Combinatorial treatment of human cardiac engineered tissues with biomimetic cues induces functional maturation as revealed by optical mapping of action potentials and calcium transients. Front Physiol. 2020;11:165.

Mehrotra S, de Melo BAG, Hirano M, Keung W, Li RA, Mandal BB, Shin S.R. Nonmulberry silk based ink for fabricating mechanically robust cardiac patches and endothelialized myocardium on-a-chip application. Funct. Mater. 2019;1907436.

Li S, Chopra A, Keung W, Chan CWY, Costa KD, Kong CW, Hajjar RJ, Chen CS, Li RA. Sarco/Endoplasmic Reticulum Ca2+-ATPase is a more effective calcium remover than Sodium-Calcium Exchanger in human embryonic stem cell-derived cardiomyocytes. Am J Physiol Heart Circ. 2019;317(5):H1105-H1115.

Chow MZ, Sadrian SN, Keung W, Geng L, Ren L, Kong CW, Wong AO, Hulot JS, Chen CS, Costa KD, Hajjar RJ, Li RA. Modulation of chromatin remodeling proteins SMYD1 and SMARCD1 promotes contractile function of human pluripotent stem cell-derived ventricular cardiomyocyte in 3D-engineered cardiac tissues. Sci Rep. 2019;9(1):7502.

Li S, Keung W, Cheng H, Li RA. Structural and mechanistic bases of nuclear calcium signaling in human pluripotent stem cell-derived ventricular cardiomyocytes. Stem Cell Int. 2019: 8765752.

Keung W, Chan PKW, Backeris PC, Lee EK, Wong N, Wong AO, Wong GK, Chan CW, Fermini B, Costa KD, Li RA. Human cardiac ventricular-like organoid chambers and tissue strips from pluripotent stem cells as a two-tiered assay for inotropic responses. Clin Pharm Ther. 2019. doi: 10.1002/cpt.1385.

Kong CW, Geng L, Li RA. High-Content Electrophysiological Analysis of Human Pluripotent Stem Cell-Derived Cardiomyocytes (hPSC-CMs). Methods Mol Biol. 2018;1722:185-194. doi: 10.1007/978-1-4939-7553-2_12.

Geng L, Kong CW, Wong AO, Shum AM, Chow MZ, Che H, Zhang C, Yau KL, Chan CW, Keung W, Li RA. Probing flecainide block of INa using human pluripotent stem cell-derived ventricular cardiomyocytes adapted to automated patch-clamping and 2D monolayers. Toxicol Lett. 2018;294:61-72.

Fu KS, Lehtinen ML, Leung CY, Lian X, Xu J, Keung W, Geng L, Kolstad TR, Thams S, Wong AO, Wong N, Bylund K, Zhou C, He X, Jin SB, Clarke J, Landahl U, Li RA, Louch WE, Chien KR. Human ISL1+ Ventricular Progenitors Self-Assemble into an In Vivo Functional Heart Patch and Preserve Cardiac Function after Infarction. Molecular Therapy. 2018;26(7):1644-1659.

Li Z, Meng Z, Lu J, Chen FM, Wong WT, Tse G, Zheng C, Keung W, Tse K, Li RA, Jiang L, Yao XJ. TRPV6 protects ER stress-induced apoptosis via ATF6α-TRPV6-JNK pathway in human embryonic stem cell-derived cardiomyocytes. Mol Cell Cardiol. 2018;120:1-11.

Li RA, Keung W, Cashman TJ, Backeris PC, Johnson BV, Bardot ES, Wong AOT, Chan PKW, Chan CWY, Costa KD. Bioengineering an electro-mechanically functional miniature ventricular heart chamber from human pluripotent stem cells. Biomaterials. 2018;163:116-127. doi: 10.1016/j.biomaterials.2018.02.024.

Lu J, Boheler KR, Jiang L, Chan CW, Tse WW, Keung W, Poon EN, Li RA, Yao X. Polycystin-2 Plays an Essential Role in Glucose Starvation-Induced Autophagy in Human Embryonic Stem Cell-Derived Cardiomyocytes. Stem Cells. 2017;36(4):501-513.

Chan PKW, Geng L, Gao Y, Keung W, Li RA. AAV-mediated conversion of human pluripotent stem cell-derived pacemaker. Biochem Biophys Res Commun. 2017 Dec 9;494(1-2):346-351. doi: 10.1016/j.bbrc.2017.10.023.

Lee EK, Tran DD, Keung W, Chan P, Wong G, Chan CW, Costa KD, Li RA, Khine M. Machine Learning of Human Pluripotent Stem Cell-Derived Engineered Cardiac Tissue Contractility for Automated Drug Classification. Stem Cell Reports. 2017 Nov 14;9(5):1560-1572. doi: 10.1016/j.stemcr.2017.09.008.

Lu J, Lee YK, Ran X, Lai WH, Li RA, Keung W, Tse K, Tse HF, Yao X. An abnormal TRPV4-related cytosolic Ca2+ rise in response to uniaxial stretch in induced pluripotent stem cells-derived cardiomyocytes from dilated cardiomyopathy patients. Biochim Biophys Acta. 2017 Nov;1863(11):2964-2972. doi: 10.1016/j.bbadis.2017.07.021.

Sun Y, Timofeyev V, Dennis A, Bektik E, Wan X, Laurita KR, Deschênes I, Li RA, Fu JD. A Singular Role of IK1 Promoting the Development of Cardiac Automaticity during Cardiomyocyte Differentiation by IK1 -Induced Activation of Pacemaker Current. Stem Cell Rev. 2017 Oct;13(5):631-643. doi: 10.1007/s12015-017-9745-1.

Tse G, Liu T, Li G, Keung W, Yeo JM, Fiona Chan YW, Yan BP, Chan YS, Wong SH, Li RA, Zhao J, Wu WKK, Wong WT. Effects of pharmacological gap junction and sodium channel blockade on S1S2 restitution properties in Langendorff-perfused mouse hearts. Oncotarget. 2017 Jul 28;8(49):85341-85352. doi: 10.18632/oncotarget.19675.

Kong CW, Chen S, Geng L, Shum AM, Sun D, Li RA. Increasing the physical size and nucleation status of human pluripotent stem cell-derived ventricular cardiomyocytes by cell fusion. Stem Cell Res. 2017 Mar;19:76-81. doi: 10.1016/j.scr.2017.01.003. Epub 2017 Jan 6.

Tse G, Liu T, Li KH, Laxton V, Wong AO, Chan YW, Keung W, Chan CW, Li RA. Tachycardia-bradycardia syndrome: Electrophysiological mechanisms and future therapeutic approaches (Review). Int J Mol Med. 2017 Mar;39(3):519-526. doi: 10.3892/ijmm.2017.2877. Epub 2017 Feb 6.

Stillitano F, Hansen J, Kong CW, Karakikes I, Funck-Brentano C, Geng L, Scott S, Reynier S, Wu M, Valogne Y, Desseaux C, Salem JE, Jeziorowska D, Zahr N, Li R, Iyengar R, Hajjar RJ, Hulot JS. Modeling susceptibility to drug-induced long QT with a panel of subject-specific induced pluripotent stem cells. Elife. 2017 Jan 30;6. pii: e19406. doi: 10.7554/eLife.19406.

Lee YT, Lin HY, Chan YW, Li KH, To OT, Yan BP, Liu T, Li G, Wong WT, Keung W, Tse G. Mouse models of atherosclerosis: a historical perspective and recent advances. Lipids Health Dis. 2017 Jan 17;16(1):12. doi: 10.1186/s12944-016-0402-5. Review.

Shum AM, Che H, Wong AO, Zhang C, Wu H, Chan CW, Costa K, Khine M, Kong CW, Li RA. A Micropatterned Human Pluripotent Stem Cell-Based Ventricular Cardiac Anisotropic Sheet for Visualizing Drug-Induced Arrhythmogenicity. Adv Mater. 2017 Jan;29(1). doi: 10.1002/adma.201602448. Epub 2016 Nov 2.

Chan PK, Li RA. Gene Delivery for the Generation of Bioartificial Pacemaker. Methods Mol Biol. 2017;1521:293-306.

Tse G, Chan YW, Keung W, Yan BP. Electrophysiological mechanisms of long and short QT syndromes. Int J Cardiol Heart Vasc. 2016 Nov 26;14:8-13. doi: 10.1016/j.ijcha.2016.11.006. eCollection 2017 Mar. Review.

Tse G, Liu T, Li KH, Laxton V, Chan YW, Keung W, Li RA, Yan BP. Electrophysiological Mechanisms of Brugada Syndrome: Insights from Pre-clinical and Clinical Studies. Front Physiol. 2016 Oct 18;7:467. eCollection 2016. Review.

Shin SR, Zhang YS, Kim DJ, Manbohi A, Avci H, Silvestri A, Aleman J, Hu N, Kilic T, Keung W, Righi M, Assawes P, Alhadrami HA, Li RA, Dokmeci MR, Khademhosseini A. Aptamer-Based Microfluidic Electrochemical Biosensor for Monitoring Cell-Secreted Trace Cardiac Biomarkers. Anal Chem. 2016 Oct 4.

Keung W, Ren L, Sen Li, Wong AO, Chopra A, Kong CW, Tomaselli GF, Chen CS, Li RA. Non-cell autonomous cues for enhanced functionality of human embryonic stem cell-derived cardiomyocytes via maturation of sarcolemmal and mitochondrial KATP channels. Sci Rep. 2016 Sep 28;6:34154. doi: 10.1038/srep34154.

Soh BS, Ng SY, Wu H, Buac K, Park JH, Lian X, Xu J, Foo KS, Felldin U, He X, Nichane M, Yang H, Bu L, Li RA, Lim B, Chien KR. Endothelin-1 supports clonal derivation and expansion of cardiovascular progenitors derived from human embryonic stem cells. Nat Commun. 2016 Mar 8;7:10774. doi: 10.1038/ncomms10774.